Inactivation of adipose angiotensinogen reduces adipose tissue macrophages and increases metabolic activity
ORCID
Latha Ramalingam: 0000-0002-4856-7327
Document Type
Article
Date
2-2016
Disciplines
Nutrition
Description/Abstract
Objective: The adipose renin-angiotensin system (RAS) has been linked to obesity-induced inflammation, though mechanisms are not completely understood. In this study, adipose-specific angiotensinogen knockout mice (Agt-KO) were generated to determine whether Agt inactivation reduces inflammation and alters the metabolic profile of the Agt-KO mice compared to wild-type (WT) littermates.
Methods: Adipose tissue-specific Agt-KO mice were created using the Cre-LoxP system with both Agt-KO and WT littermates fed either a low-fat or high-fat diet to assess metabolic changes. White adipose tissue was used for gene/protein expression analyses and WAT stromal vascular cells for metabolic extracellular flux assays.
Results: No significant differences were observed in body weight or fat mass between both genotypes on either diet. However, improved glucose clearance was observed in Agt-KO compared to WT littermates, consistent with higher expression of genes involved in insulin signaling, glucose transport, and fatty acid metabolism. Furthermore, Agt inactivation reduced total macrophage infiltration in Agt-KO mice fed both diets. Lastly, stroma vascular cells from Agt-KO mice revealed higher metabolic activity compared to WT mice.
Conclusions: These findings indicate that adipose-specific Agt inactivation leads to reduced adipose inflammation and increased glucose tolerance mediated in part via increased metabolic activity of adipose cells.
Recommended Citation
LeMieux MJ, Ramalingam L, Mynatt RL, Kalupahana NS, Kim JH, Moustaïd-Moussa N. Inactivation of adipose angiotensinogen reduces adipose tissue macrophages and increases metabolic activity. Obesity (Silver Spring). 2016 Feb;24(2):359-67. doi: 10.1002/oby.21352. Epub 2015 Dec 25. PMID: 26704350.
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