Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice
ORCID
Latha Ramalingam: 0000-0002-4856-7327
Document Type
Article
Date
5-2020
Keywords
dose-response, eicosapentaenoic acid, glucose homeostasis, mice, obesity, omega 3 fatty acids
Disciplines
Nutrition
Description/Abstract
Obesity is a widespread epidemic that increases the risk for several metabolic diseases. Despite several beneficial health effects of eicosapentaenoic acid (C20:5n-3, EPA), previous studies have used very high doses of EPA. In this study, dose-dependent effects of EPA on metabolic outcomes were determined in diet-induced obese mice. We used B6 male mice, fed high-fat diet (HF, 45% kcal fat) or HF diet supplemented with 9, 18, and 36 g/kg of EPA-enriched fish oil for 14 weeks. We conducted metabolic phenotyping during the feeding period, and harvested tissues and blood at termination. Only mice fed 36 g/kg of EPA significantly (p < 0.05) lowered body weight, fat content and epididymal fat pad weight, compared to HF. Both 18 and 36 g/kg doses of EPA significantly increased glucose clearance and insulin sensitivity, compared to HF or 9 g/kg of EPA. Locomotor activity was significantly increased with both 18 and 36 g/kg doses of EPA. Interestingly, all doses of EPA compared to HF, significantly increased energy expenditure and oxygen consumption and significantly reduced serum insulin, leptin, and triglycerides levels. These results demonstrate weight- and adiposity-independent metabolic benefits of EPA, at doses comparable to those currently used to treat hypertriglyceridemia.
Recommended Citation
Pahlavani M, Ramalingam L, Miller EK, Davis H, Scoggin S, Moustaid-Moussa N. Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice. Nutrients. 2020 May 8;12(5):1342. doi: 10.3390/nu12051342. PMID: 32397139; PMCID: PMC7284763.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
