Document Type

Honors Capstone Project

Date of Submission

Spring 5-1-2011

Capstone Advisor

Prof. Rob Doyle

Honors Reader

Prof. Jon Zubieta

Capstone Major

Chemistry

Capstone College

Arts and Science

Audio/Visual Component

no

Capstone Prize Winner

no

Won Capstone Funding

yes

Honors Categories

Sciences and Engineering

Subject Categories

Chemistry | Organic Chemistry

Abstract

This project aimed to create a novel oral tetanus vaccine by exploiting the B12 uptake pathway. While many linkers were attempted, the only successful complex was created using CDT coupling. After synthesis, purification (with some difficulty), and characterization, tests were done in vitro and in vivo. These supported the conclusion that while the complex was being successfully taken up by the cell, the complex was not created a strong enough immune response for a positive titer. On a second note, simply the uptake of B12-TT is remarkable because it is the largest molecule that has been taken up via cubilin-mediated endocytosis. It is five times larger than the previously established largest molecule carried by B12 and is a great stride for scientists considering the B family vitamins as drug delivery systems.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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