Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2011
Capstone Advisor
Prof. Rob Doyle
Honors Reader
Prof. Jon Zubieta
Capstone Major
Chemistry
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
yes
Honors Categories
Sciences and Engineering
Subject Categories
Chemistry | Organic Chemistry
Abstract
This project aimed to create a novel oral tetanus vaccine by exploiting the B12 uptake pathway. While many linkers were attempted, the only successful complex was created using CDT coupling. After synthesis, purification (with some difficulty), and characterization, tests were done in vitro and in vivo. These supported the conclusion that while the complex was being successfully taken up by the cell, the complex was not created a strong enough immune response for a positive titer. On a second note, simply the uptake of B12-TT is remarkable because it is the largest molecule that has been taken up via cubilin-mediated endocytosis. It is five times larger than the previously established largest molecule carried by B12 and is a great stride for scientists considering the B family vitamins as drug delivery systems.
Recommended Citation
Soldner, Teresa, "Exploring the Potential for B12-based Oral Vaccine Delivery; Cubilin mediated uptake of B12-conjugate systems greater than 160 kDa" (2011). Renée Crown University Honors Thesis Projects - All. 261.
https://surface.syr.edu/honors_capstone/261
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
