Date of Award
8-4-2023
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Biomedical and Chemical Engineering
Advisor(s)
Juntao Luo
Second Advisor
Ian Hosein
Keywords
Antibiotics;Daptomycin;Drug Delivery;Nanopaticles;Telodendrimer
Subject Categories
Life Sciences | Pharmacology | Pharmacology, Toxicology and Environmental Health
Abstract
The immune system plays a crucial role in fighting pathogens, but infections can occur if the immune system is unable to overcome them, leading to severe damage and death. Antibiotics and drug delivery techniques have helped save countless lives and improve the quality of life for patients. Daptomycin (Dap) is a powerful antibiotic used to treat gram-positive bacterial infections. However, its hydrophobic nature limits its effectiveness, and it requires the presence of calcium ions (Ca2+) for activation. In this study, a series of telodendrimer (TD) nanoparticle delivery systems were synthesized to improve daptomycin delivery. The TD nanoparticle systems showed effective binding with daptomycin, forming Dap-TD nanoparticles. Further more, this binding behavior between daptomycin and Ca2+ was examined via fluorescence spectrum, and indicated that TD can protection daptomycin via encapsulation to achieve broader distribution in the circulation. Dap-TDs demonstrated superior antibacterial activity against staphylococcus and streptococcus strains, while showing weaker but acceptable inhibition against listeria. Cell uptake studies showed efficient membrane penetration of the Dap-TDs, and the cytotoxicity levels, hemolytic effect were low or acceptable for some nanoformulation. These findings highlight the potential of telodendrimers as an effective drug delivery system for daptomycin and their capability to bind with bacterial remnants after bacterial cell death. This knowledge contributes valuable insights for the development of enhanced therapeutic approaches against Gram-positive bacterial infections.
Recommended Citation
Yue, Hao, "Development Of Telodendrimer Nanocarriers For Daptomycin Delivery" (2023). Theses - ALL. 765.
https://surface.syr.edu/thesis/765
