ORCID
Alison E. Patteson: 0000-0002-4004-1734
Maxx Swoger: 0000-0001-6128-8704
Nikhila Krishnan: 0000-0001-8995-6946
Judy Freshour: 0000-0003-3235-093X
Heidi Henley: 0000-0001-6660-5254
Document Type
Article
Date
Spring 3-18-2022
Language
English
Funder(s)
National Institutes of Health, Syracuse University BioInspired Seed Grant, the US Army Medical Research Acquisition Activity
Funding ID
R35GM142963, R01GM127621, R01GM130874, NIH S10 OD026946-01A1, W81XWH-20-1-0585
Acknowledgements
We thank Li-En Jao lab (UCSD) for the Tg(pcnttup2) transgenic zebrafish lines and genotyping protocols for characterization. This work was supported by National Institutes of Health grants R35GM142963 (AE Patteson), R01GM127621 (H Hehnly) and R01GM130874 (H Hehnly), and the Syracuse University BioInspired Seed Grant (AE Patteson and H Hehnly). We thank the Blatt BioImaging Center for the use of the LSM 980 with Airyscan 2 (NIH S10 OD026946-01A1). This work was also supported by the US Army Medical Research Acquisition Activity through the FY16 Prostate Cancer Research Programs under Award no. W81XWH-20-1-0585 (H Hehnly). Opinions, interpretations, conclusions, and recommendations are those of the authors and not necessarily endorsed by the Department of Defense.
Official Citation
Krishnan N, Swoger M, Rathbun LI, Fioramonti PJ, Freshour J, Bates M, Patteson AE, Hehnly H. Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo. Life Sci Alliance. 2022 Mar 18;5(7):e202201362. doi: 10.26508/lsa.202201362. PMID: 35304423; PMCID: PMC8933627.
Disciplines
Physics
Description/Abstract
The last stage of cell division involves two daughter cells remaining interconnected by a cytokinetic bridge that is cleaved during abscission. Conserved between the zebrafish embryo and human cells, we found that the oldest centrosome moves in a Rab11-dependent manner towards the cytokinetic bridge sometimes followed by the youngest. Rab11-endosomes are organized in a Rab11-GTP dependent manner at the mother centriole during pre-abscission, with Rab11 endosomes at the oldest centrosome being more mobile compared with the youngest. The GTPase activity of Rab11 is necessary for the centrosome protein, Pericentrin, to be enriched at the centrosome. Reduction in Pericentrin expression or optogenetic disruption of Rab11-endosome function inhibited both centrosome movement towards the cytokinetic bridge and abscission, resulting in daughter cells prone to being binucleated and/or having supernumerary centrosomes. These studies suggest that Rab11-endosomes contribute to centrosome function during pre-abscission by regulating Pericentrin organization resulting in appropriate centrosome movement towards the cytokinetic bridge and subsequent abscission.
Recommended Citation
Krishnan N, Swoger M, Rathbun LI, Fioramonti PJ, Freshour J, Bates M, Patteson AE, Hehnly H. Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo. Life Sci Alliance. 2022 Mar 18;5(7):e202201362. doi: 10.26508/lsa.202201362. PMID: 35304423; PMCID: PMC8933627.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
