Bound Volume Number

Volume II

Document Type

Honors Capstone Project

Date of Submission

Spring 5-2016

Capstone Advisor

Ramesh Raina

Capstone Major

Biology

Capstone College

Arts and Science

Audio/Visual Component

no

Keywords

JMJ3, Arabidopsis thaliana

Capstone Prize Winner

no

Won Capstone Funding

no

Honors Categories

Sciences and Engineering

Subject Categories

Biology

Abstract

The Raina lab studies plant-pathogen interactions through genetics and molecular biology by using the model organism, Arabidopsis thaliana. A group of genes in A. thaliana, the Jumonji gene family, regulates gene expression by epigenetic mechanisms. Jumonji genes influence histone demethylation. Previous research in the Raina lab has shown that some Jumonji (JMJ) genes play a role in immune signaling in A. thaliana in response to pathogens. My project is to characterize JMJ3 gene, and see if there was any role that JMJ3 played in pathogen defense in A. thaliana.

The goal of my study was to investigate the possible role of JMJ3 in pathogen defense. To assess this, jmj3-1 and jmj3-3 mutant lines were challenged with the bacterial pathogen, Pseudomonas syringae, and the fungal pathogen, Botrytis cinerea, and pathogen growth in these mutants was determined. These mutant plants were compared to wild type plants (WT) to determine if there was any change in disease susceptibility due to loss of function of this gene. My results indicate that JMJ3 negatively regulates defense against Pseudomonas syringae.

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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Biology Commons

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