Bound Volume Number
Volume II
Degree Type
Honors Capstone Project
Date of Submission
Spring 5-2016
Capstone Advisor
Ramesh Raina
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Keywords
JMJ3, Arabidopsis thaliana
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biology
Abstract
The Raina lab studies plant-pathogen interactions through genetics and molecular biology by using the model organism, Arabidopsis thaliana. A group of genes in A. thaliana, the Jumonji gene family, regulates gene expression by epigenetic mechanisms. Jumonji genes influence histone demethylation. Previous research in the Raina lab has shown that some Jumonji (JMJ) genes play a role in immune signaling in A. thaliana in response to pathogens. My project is to characterize JMJ3 gene, and see if there was any role that JMJ3 played in pathogen defense in A. thaliana.
The goal of my study was to investigate the possible role of JMJ3 in pathogen defense. To assess this, jmj3-1 and jmj3-3 mutant lines were challenged with the bacterial pathogen, Pseudomonas syringae, and the fungal pathogen, Botrytis cinerea, and pathogen growth in these mutants was determined. These mutant plants were compared to wild type plants (WT) to determine if there was any change in disease susceptibility due to loss of function of this gene. My results indicate that JMJ3 negatively regulates defense against Pseudomonas syringae.
Recommended Citation
Trysler, Nelson, "Molecular Mechanisms Regulating Pathogen Defense: The Role of JMJ3 in Arabidopsis thaliana" (2016). Honors Capstone Projects - All. 930.
https://surface.syr.edu/honors_capstone/930
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
