Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2013
Capstone Advisor
Dr. James Hougland
Honors Reader
Dr. James Dabrowiak
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
yes
Honors Categories
Sciences and Engineering
Subject Categories
Biochemistry
Abstract
Ghrelin is a peptide hormone involved in hunger signaling and other physiological processes. To become active, ghrelin first must be acylated by ghrelin O-acyltransferase (GOAT). This enzyme carries out a post-translational modification that attaches an octanoyl group to the side chain of the serine 3 (S3) residue of ghrelin. With ghrelin linked to hunger signaling, memory, and other physiological processes, GOAT has been identified as a potential drug target. However, inhibitor design is difficult because not much is known regarding the active site structure and catalytic mechanism of GOAT. This study reports the design of a novel fluorescence-based assay that allows the use of ghrelin substrate mimics and derivatives to interrogate ghrelin recognition by GOAT. The results of this research can directly contribute to helping in the development of novel GOAT inhibitors.
Recommended Citation
Prybolsky, Edward P., "Investigation of ghrelin acylation by ghrelin O-acyltransferase" (2013). Renée Crown University Honors Thesis Projects - All. 69.
https://surface.syr.edu/honors_capstone/69
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