Document Type

Honors Capstone Project

Date of Submission

Spring 5-1-2006

Capstone Advisor

Rachel Charron

Honors Reader

Dr. John Belote

Capstone Major

Biology

Capstone College

Arts and Science

Audio/Visual Component

no

Capstone Prize Winner

no

Won Capstone Funding

no

Honors Categories

Sciences and Engineering

Subject Categories

Biology

Abstract

The study of genomic analysis of deletion mutants in Saccharomyces cerevisiae associated with mitochondrial genome stability has produced both genetic and biochemical findings to be applied to the study of many mitochondrial genetic disorders in humans. Saccharomyces cerevisiae has approximately 6,200 ORFs in its genome. Of the 6,200 ORFs, 4,878 are non-essential. The EuroScarf deletion project removed all the non-essential ORFs, of the 4,878 viable haploid strains of Saccharomyces cerevisiae, and replaced them with the G418 resistance cassette. A subset of 700 deletion mutants that were previously identified as important for mitochondrial function and biogenesis was compiled from the EuroScarf knockout library. The deletion set was analyzed on various non-fermentable and fermentable carbon sources for differential growth patterns. The rate of petite formation was measured on the various medias. The deletion library was then screened onto YPD + ethidium bromide, turning all the genes into rho° cells. The screen was conducted to measure the resistance of the deletion set to petite formation. Lastly was the comparison of the growth of the intronless strain JC25-Mit-I compared to the growth of the intron strain JC25-wt on varying medias, such as SCG and SG5FOA. Twenty-five genes were identified to be important in splicing in mitochondria. The data indicated both an indirect and a direct relationship between introns and the formation and growth of petites. The research conducted in the three experiments concerning the genomic analysis of yeast deletion mutants associated with mitochondrial genome stability have helped to lay the framework for future research. Mitochondrial genetic disorders in humans are cause for great concern, but due to the genetic tractability between yeast and human cells, much of the findings discovered in yeast can be applied to humans.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Biology Commons

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