Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2006
Capstone Advisor
Rachel Charron
Honors Reader
Dr. John Belote
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biology
Abstract
The study of genomic analysis of deletion mutants in Saccharomyces cerevisiae associated with mitochondrial genome stability has produced both genetic and biochemical findings to be applied to the study of many mitochondrial genetic disorders in humans. Saccharomyces cerevisiae has approximately 6,200 ORFs in its genome. Of the 6,200 ORFs, 4,878 are non-essential. The EuroScarf deletion project removed all the non-essential ORFs, of the 4,878 viable haploid strains of Saccharomyces cerevisiae, and replaced them with the G418 resistance cassette. A subset of 700 deletion mutants that were previously identified as important for mitochondrial function and biogenesis was compiled from the EuroScarf knockout library. The deletion set was analyzed on various non-fermentable and fermentable carbon sources for differential growth patterns. The rate of petite formation was measured on the various medias. The deletion library was then screened onto YPD + ethidium bromide, turning all the genes into rho° cells. The screen was conducted to measure the resistance of the deletion set to petite formation. Lastly was the comparison of the growth of the intronless strain JC25-Mit-I compared to the growth of the intron strain JC25-wt on varying medias, such as SCG and SG5FOA. Twenty-five genes were identified to be important in splicing in mitochondria. The data indicated both an indirect and a direct relationship between introns and the formation and growth of petites. The research conducted in the three experiments concerning the genomic analysis of yeast deletion mutants associated with mitochondrial genome stability have helped to lay the framework for future research. Mitochondrial genetic disorders in humans are cause for great concern, but due to the genetic tractability between yeast and human cells, much of the findings discovered in yeast can be applied to humans.
Recommended Citation
Charron, Rachel, "Gene Deletion in Yeast that Affects Mitochondrial DNA Stability, Function and Maintenance" (2006). Renée Crown University Honors Thesis Projects - All. 627.
https://surface.syr.edu/honors_capstone/627
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