Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2013
Capstone Advisor
James C Dabrowiak, Ph.D.
Honors Reader
Surabhi Raina, Ph.D.
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biochemistry | Biology | Chemistry
Abstract
The transcription factor p53 can induce apoptosis and cell cycle arrest in response to cellular distress. Cancer cells often display increased cell survival. In most cases, this is due to a p53-related defect, such as mutation, deletion, degradation, or sequestration. HDM2 and HDMX are homologous proteins that regulate the function of p53, and their over-expression can lead to an ineffective p53 response. Various inhibitors, including hydrocarbon stabilized alpha-helices of p53 (SAH-p53s), have been developed to target HDM2 and HDMX and restore functionality to the p53 pathway. It has been recently found that SAH-p53 factors also elicit cell death responses in the absence of transcriptionally active p53. In order to investigate other potential targets of SAH-p53, a protocol was developed based on a paper by Craig Braun 11 which discusses a mechanism utilizing photoactivatable amino substitutions to capture binding partners covalently. Using this protocol, p53-derived stapled peptides were found to bind selectively to p53’s known target, HDM2 as well as other unknown proteins. The various p53-derived stapled peptides were utilized to obtain further insight into the p53-derived stapled peptide targets.
Recommended Citation
Headley, Kathryn Margaret, "The Use of p53-Derived Stapled Peptides as Affinity Isolation Reagents" (2013). Renée Crown University Honors Thesis Projects - All. 61.
https://surface.syr.edu/honors_capstone/61
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
