Honors Capstone Project
Date of Submission
Scott E. Erdman
Arts and Science
Capstone Prize Winner
Won Capstone Funding
Sciences and Engineering
Biochemistry | Biochemistry, Biophysics, and Structural Biology
Pseudomonas aeruginosa mutants PA0686, PA3102, PA3103, and PA5210 carry transposon insertions in uncharacterized genes in the PA01 background. The biofilms formed by these mutants show different phenotypes than PA01, their wild-type counterpart. BLAST searches and phylogenomic mapping tools indicate that protein products of the mutated genes are involved in P. aeruginosa type-II secretion, which is linked to twitching motility. In this study, 96-well microtitre plate biofilm assays, UV/visible spectrophotometer analysis, and motility assays were used to demonstrate a link between type-II secretion, twitching motility and biofilm formation. The results show that the PA5210 mutant forms a biofilm that is less compact than the wild-type, while PA0686 forms a faint biofilm. Also, the motility assays support our hypothesis that these mutants are defective in twitching motility.
Suh, Sijung, "Characterization of Type II secretion mutants of Pseudomonas aeruginosa" (2007). Honors Capstone Projects - All. 577.
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