Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2007
Capstone Advisor
Anthony Garza
Honors Reader
Scott E. Erdman
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biochemistry | Biochemistry, Biophysics, and Structural Biology
Abstract
Pseudomonas aeruginosa mutants PA0686, PA3102, PA3103, and PA5210 carry transposon insertions in uncharacterized genes in the PA01 background. The biofilms formed by these mutants show different phenotypes than PA01, their wild-type counterpart. BLAST searches and phylogenomic mapping tools indicate that protein products of the mutated genes are involved in P. aeruginosa type-II secretion, which is linked to twitching motility. In this study, 96-well microtitre plate biofilm assays, UV/visible spectrophotometer analysis, and motility assays were used to demonstrate a link between type-II secretion, twitching motility and biofilm formation. The results show that the PA5210 mutant forms a biofilm that is less compact than the wild-type, while PA0686 forms a faint biofilm. Also, the motility assays support our hypothesis that these mutants are defective in twitching motility.
Recommended Citation
Suh, Sijung, "Characterization of Type II secretion mutants of Pseudomonas aeruginosa" (2007). Renée Crown University Honors Thesis Projects - All. 577.
https://surface.syr.edu/honors_capstone/577
Creative Commons License
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