Document Type

Honors Capstone Project

Date of Submission

Spring 5-1-2007

Capstone Advisor

Ramesh Raina

Honors Reader

John Belote

Capstone Major

Biology

Capstone College

Arts and Science

Audio/Visual Component

no

Capstone Prize Winner

no

Won Capstone Funding

no

Honors Categories

Sciences and Engineering

Subject Categories

Biology

Abstract

Arabidopsis thaliana is an excellent model plant to study various plant processes, including plant’s response to environment, its interactions with other organisms, etc. In this study I characterized eight Arabidopsis genes that are likely to regulate plant’s responses to biotic and abiotic stresses. Microarray analysis was done previously in our lab to identify Arabidopsis genes that are differentially expressed in response to various biotic and abiotic stresses and several plant hormones. The biotic stresses include bacterial, fungal and viral pathogens (both virulent and avirulent strains), sucking and chewing insects. The abiotic stresses include chemicals that induce variety of oxidative stresses (paraquat, 3AT, mixture of glucose/glucose oxidase, and mixture of xanthine/xantine), wounding, heat, cold, freezing, senescence, drought, flooding and salt. The plant hormones include IAA (auxin), 2,4-D (synthetic auxin), BA (cytokinin), GA (gibberellic acid) ,ABA (abscisic acid) , ACC (ethylene precursor), JA (jasmonic acid), BR (brassinosteroid), and SA (salicylic acid). The results of these experiments were used to construct Arabidopsis Stress Microarray Database (ASMD). From this database, eight genes that were considerably up regulated in a number of different stress treatments were identified. To determine the role of these genes in regulating stresses, I constructed transgenic plants that over- or under-express the target genes.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Biology Commons

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