Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2007
Capstone Advisor
Ramesh Raina
Honors Reader
John Belote
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biology
Abstract
Arabidopsis thaliana is an excellent model plant to study various plant processes, including plant’s response to environment, its interactions with other organisms, etc. In this study I characterized eight Arabidopsis genes that are likely to regulate plant’s responses to biotic and abiotic stresses. Microarray analysis was done previously in our lab to identify Arabidopsis genes that are differentially expressed in response to various biotic and abiotic stresses and several plant hormones. The biotic stresses include bacterial, fungal and viral pathogens (both virulent and avirulent strains), sucking and chewing insects. The abiotic stresses include chemicals that induce variety of oxidative stresses (paraquat, 3AT, mixture of glucose/glucose oxidase, and mixture of xanthine/xantine), wounding, heat, cold, freezing, senescence, drought, flooding and salt. The plant hormones include IAA (auxin), 2,4-D (synthetic auxin), BA (cytokinin), GA (gibberellic acid) ,ABA (abscisic acid) , ACC (ethylene precursor), JA (jasmonic acid), BR (brassinosteroid), and SA (salicylic acid). The results of these experiments were used to construct Arabidopsis Stress Microarray Database (ASMD). From this database, eight genes that were considerably up regulated in a number of different stress treatments were identified. To determine the role of these genes in regulating stresses, I constructed transgenic plants that over- or under-express the target genes.
Recommended Citation
Whitlock, Jessica M., "Molecular Analysis of Arabidopsis thaliana Genes Involved in Stress Response" (2007). Renée Crown University Honors Thesis Projects - All. 576.
https://surface.syr.edu/honors_capstone/576
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