Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2008
Capstone Advisor
Dr. James C. Dabrowiak
Honors Reader
Dr. Jerry Goodisman
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biochemistry | Biochemistry, Biophysics, and Structural Biology
Abstract
The reaction of aged carboplatin (reaction of carboplatin in 24 mM NaHCO3 for 45 h, 37o C, pH 8.6) with pBR322 DNA for 0 ≤ r ≤ 2.8, where r = [drug]/[DNA-bp], in 24 mM HEPES buffer, pH 7.4, for 24 h followed by agarose gel electrophoresis showed DNA mobility changes consistent with unwinding closed circular DNA. However, identical experiments conducted in a two buffer system, 24mM HEPES plus 24mM carbonate, showed no DNA mobility changes, indicating that carbonate blocks formation of the 1,2-intrastrand crosslink on DNA. Studies with aged carboplatin and with cisplatin carried out with 2.0 ≤ r ≤ 10.0 in the two buffer system show that some DNA binding and unwinding occurs for both drugs. Since carbonate inhibits the binding of aged carboplatin and cisplatin to DNA, carbonate present in the body likely modulates the reactivity of these drugs with a variety of biological targets including DNA.
Recommended Citation
Sorokanich, Robert S., "Influence of Carbonate on the Binding of Carboplatin to DNA" (2008). Renée Crown University Honors Thesis Projects - All. 525.
https://surface.syr.edu/honors_capstone/525
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