Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2011
Capstone Advisor
Mark E. Schmitt, Ph.D.
Honors Reader
William T. Starmer, Ph.D.
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biology | Ecology and Evolutionary Biology
Abstract
The yeast, Saccharomyces cerevisiae, is a eukaryote and model organism that can be used to study how eukaryotic cells respond to hypergravity. In this experiment, six standard wild-type strains were used to determine a maximum gravitational force the yeast can withstand and still grow. Artificial gravity was generated using a centrifuge. Yeasts were found to maintain growth even at the highest artificial gravity tested, 2,114 g. An ordered deletion set of S. cerevisiae was then screened to identify genes that could not grow and withstand simulated hypergravity compared to growth under 1g conditions. Many of the strains that failed to grow at high gravity were found to be related to mitochondrial gene expression and translation. Mitochondria, the powerhouse and ATP source of the cell, are large organelles involved in fatty acid oxidation, the citric acid cycle, and respiration. Gene localization in the mitochondria is important in studying mitochondrial pathways, and intracellular organization such as mitochondrial fusion, vesicular docking, and protein trafficking.
Recommended Citation
Vangruber, Sapir, "Growth of Yeast, Saccharomyces cerevisiae, under Hypergravity Conditions" (2011). Renée Crown University Honors Thesis Projects - All. 258.
https://surface.syr.edu/honors_capstone/258
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
