Author

Jessica Cho

Document Type

Honors Capstone Project

Date of Submission

Spring 5-1-2011

Capstone Advisor

Dr. David Amberg

Honors Reader

Dr. James Dabrowiak

Capstone Major

Biology

Capstone College

Arts and Science

Audio/Visual Component

no

Capstone Prize Winner

no

Won Capstone Funding

no

Honors Categories

Sciences and Engineering

Subject Categories

Biochemistry | Biochemistry, Biophysics, and Structural Biology | Chemistry

Abstract

The actin cytoskeleton is a vitally important organizing structure found in the cytoplasm of our cells. It contributes to essential cell functions from growth and cell division, development, signal transduction, adaptation, to gene expression. Interest in how regulation of the actin cytoskeleton---due to actin cytoskeleton's importance to cell function-may contribute to diverse human disease states such as the rampant cell division and metastasis that occurs in cancerous tissues. It has been further elucidated that many human genetic disorders are the result of complex haploinsufficiencies (CHI) and/or altered gene dosages at multiple loci. By reviewing a large number of potential Synthetic Dosage Lethality (SDL) interactions with the yeast actin gene ACT1 initially identified in robotic screens, a smaller set of genes were reviewed, compiled, and retested with plasmid transformation tests. Then the list was expanded to include genes within the same complex or with the same functions using Gene Ontology terms. The implicated gene set-particularly elongator/urmylation/tRNA-modification sets of overlapping genes- was followed up with further tests to find the most relevant complexes or functions to the observed ACT1 SDL. Further tests include rhodamine-phalloidin actin stain and TECAN runs.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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