Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2011
Capstone Advisor
Dr. David Amberg
Honors Reader
Dr. James Dabrowiak
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biochemistry | Biochemistry, Biophysics, and Structural Biology | Chemistry
Abstract
The actin cytoskeleton is a vitally important organizing structure found in the cytoplasm of our cells. It contributes to essential cell functions from growth and cell division, development, signal transduction, adaptation, to gene expression. Interest in how regulation of the actin cytoskeleton---due to actin cytoskeleton's importance to cell function-may contribute to diverse human disease states such as the rampant cell division and metastasis that occurs in cancerous tissues. It has been further elucidated that many human genetic disorders are the result of complex haploinsufficiencies (CHI) and/or altered gene dosages at multiple loci. By reviewing a large number of potential Synthetic Dosage Lethality (SDL) interactions with the yeast actin gene ACT1 initially identified in robotic screens, a smaller set of genes were reviewed, compiled, and retested with plasmid transformation tests. Then the list was expanded to include genes within the same complex or with the same functions using Gene Ontology terms. The implicated gene set-particularly elongator/urmylation/tRNA-modification sets of overlapping genes- was followed up with further tests to find the most relevant complexes or functions to the observed ACT1 SDL. Further tests include rhodamine-phalloidin actin stain and TECAN runs.
Recommended Citation
Cho, Jessica, "Synthetic Dosage Lethality Screen with Actin Cytoskeleton in Yeast" (2011). Renée Crown University Honors Thesis Projects - All. 250.
https://surface.syr.edu/honors_capstone/250
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
