Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2011
Capstone Advisor
Dr. Robert Doyle
Honors Reader
Dr. Samual Chan
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
yes
Honors Categories
Sciences and Engineering
Subject Categories
Biochemistry | Biochemistry, Biophysics, and Structural Biology
Abstract
The growing number of strains of antibiotic-resistant bacteria poses a new threat to human health. The development of innovative strategies to treat bacterial infections is essential. The recent discovery of a new class of membrane transporters, CitMHS, unique to bacteria, presents a potential new target for antibacterial therapeutics. CitMHS is a class of secondary metal citrate membrane transporters, with only five members characterized to date (2,3,5,6,7). No crystal structures have been reported. Detailed structural and functional studies of CitMHS are essential to the understanding of infection pathways and, consequently, the ability to block such pathways. CitBa is a CitMHS transporter postulated in the Gram-positive bacterium, Bacillus anthracis, which is the causative agent of anthrax. Heterologous expression of CitBa in Lactococcus lactis following functional flux assays may reveal its metal citrate transport capability. Additionally, protein purification may enable antibody production, which may then be used as a vaccine or vaccine component for anthrax.
Recommended Citation
Li, Qi Wen, "Structural and Functional Characterization of a Putative CitMHS Protein from Bacillus anthracis" (2011). Renée Crown University Honors Thesis Projects - All. 244.
https://surface.syr.edu/honors_capstone/244
Capstone Part 2
Capstone part III.docx (16 kB)
Capstone Part 3
Capstone part IV.docx (781 kB)
Capstone Part 4
Protein Sequences.docx (13 kB)
Protein Sequences
Radiation 01-24-2011.pdf (96 kB)
Radiation 01-24-2011
Radiation 01-27-2011.pdf (95 kB)
Radiation 01-27-2011
Radiation 02-01-2011.pdf (164 kB)
02-01-2011
pET-25b(+).pdf (39 kB)
pET-25b(+)
pFLAG-CTS.pdf (220 kB)
Creative Commons License
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