Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2018
Capstone Advisor
Jeffrey Amack
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biology | Life Sciences
Abstract
Axenfeld-Rieger Syndrome (ARS) has been shown to be correlated with the Pitx2 mutation in both humans and zebrafish (Ji et al, 2016). ARS is classified as a rare autosomal dominant disorder that includes defects in the eyes, teeth and craniofacial design (Dressler et al 2010). The earliest signs of a Pitx2 mutation have yet to be defined in tooth morphogenesis. In this review, the earliest signs of the mutation are defined at 4dpf but, it is understood that further research needs to be done in order to classify that this mutation does not start to appear earlier.
The second goal was to analyze expression of candidate genes that were found, specifically sidekey269.114, MyHz2 and CyP4v7, in pitx2 mutants in order to see if certain gene expressions are changed. Using In Situ Hybridization (ISH) experiments, it was found that the sidekey269.114 gene may show early signs of expression or increased expression in pitx2 mutants. No mutants were found for MyHz2 and CyP4v7. No obvious differences in expression were seen between heterozygous (HET) and wild-type (WT) pitx2 embryos.
Recommended Citation
Stylianou, Anthe, "Characterizing Pitx2 Tooth Morphology and Candidate Genes of Axenfeld- Rieger Syndrome in Zebrafish" (2018). Renée Crown University Honors Thesis Projects - All. 1166.
https://surface.syr.edu/honors_capstone/1166
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