Document Type

Honors Capstone Project

Date of Submission

Spring 5-2017

Capstone Advisor

John Belote

Honors Reader

Heather Coleman

Capstone Major

Biology

Capstone College

Arts and Science

Audio/Visual Component

no

Capstone Prize Winner

no

Won Capstone Funding

no

Honors Categories

Sciences and Engineering

Subject Categories

Biology

Abstract

The goal of the project was to develop a tool that allows for targeted expression of genes in spermatogenesis. To do this the GAL4/UAS system was employed. This system places the coding region of the GAL4 gene under a tissue specific promoter, ProtamineB in this case. The construct is transformed into Drosophila melanogaster and crossed with an existing UAS-reporter strain to make visualization of successful transformants easy. Expression was searched for in the testes by dissection and various detection methods. Unfortunately no expression was seen in the testes despite the construct being successfully integrated into the genome. This information tells us about the timing of expression within late spermatogenesis, and that a tool can successfully be developed to target genes of interest within spermatogenesis.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

Included in

Biology Commons

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