Degree Type
Honors Capstone Project
Date of Submission
Spring 5-2017
Capstone Advisor
John Belote
Honors Reader
Heather Coleman
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
no
Won Capstone Funding
no
Honors Categories
Sciences and Engineering
Subject Categories
Biology
Abstract
The goal of the project was to develop a tool that allows for targeted expression of genes in spermatogenesis. To do this the GAL4/UAS system was employed. This system places the coding region of the GAL4 gene under a tissue specific promoter, ProtamineB in this case. The construct is transformed into Drosophila melanogaster and crossed with an existing UAS-reporter strain to make visualization of successful transformants easy. Expression was searched for in the testes by dissection and various detection methods. Unfortunately no expression was seen in the testes despite the construct being successfully integrated into the genome. This information tells us about the timing of expression within late spermatogenesis, and that a tool can successfully be developed to target genes of interest within spermatogenesis.
Recommended Citation
Figaniak, Jake, "Development of a Spermatogenesis Specific Gene Driver Using the UAS-GAL4 System" (2017). Renée Crown University Honors Thesis Projects - All. 1056.
https://surface.syr.edu/honors_capstone/1056
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
