Date of Award

5-12-2024

Degree Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Biomedical and Chemical Engineering

Advisor(s)

Zhen Ma

Keywords

cardiac organoids;development;drug screening;IPSC;PEG

Abstract

Nowadays 9 of every 10 women take at least one medicine during their pregnancy. However, unfortunately, many pregnant women have to face a severe problem that there is not enough evidence to prove the safety of their medication, and an appropriate medication might cause birth defects or even infant death. Researchers need an accurate and reliable drug developmental platform. Compared to the traditional animal and cell models, organoids have the ability to highly mimic the human organ developmental process, which allow researchers investigate the developmental toxicity to human development with organoid platform. The goal of this work is to explore the potential of cardiac organoid models to investigate heart development and cardiac function. In this work, we have utilized different patterns, including circles, squares and rectangles, and pentagrams, to create the geometrical confinement to cardiac organoids development, which is shown as having different functional properties and morphology due to the geometrical designs. We also tested the developmental toxicity of 14 drugs or chemical compounds with 600-micron circle organoids. Cardiac organoid development was interfered with the high-concentration drug treatment. These new cardiac organoids were utilized as potential advancements in drug screening applications for better predictions of drug-related developmental toxicity.

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Open Access

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