Title

Quantitative Footprinting Analysis. Binding to a Single Site

Author(s)/Creator(s)

Robert Rehfuss, Syracuse University
Jerry Goodisman, Syracuse UniversityFollow
James C. Dabrowiak, Syracuse UniversityFollow

Document Type

Article

Date

8-24-1989

Keywords

Chemistry

Disciplines

Chemistry

Description/Abstract

The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I.

Additional Information

Reprinted with permission from Rehfuss, R., Goodisman, J., & Dabrowiak, J. C. (1990). Quantitative footprinting analysis. binding to a single site. Biochemistry, 29(3), 777-781. Copyright 1990 American Chemical Society.

http://pubs.acs.org/doi/pdf/10.1021/bi00455a027

Recommended Citation

Rehfuss, R., Goodisman, J., & Dabrowiak, J. C. (1990). Quantitative footprinting analysis. binding to a single site. Biochemistry, 29(3), 777-781.

Source

Local input

Creative Commons License

This work is licensed under a Creative Commons Attribution 3.0 License.