Degree Type
Honors Capstone Project
Date of Submission
Spring 5-1-2010
Capstone Advisor
Dr. Robert Doyle
Honors Reader
Dr. Jon Zubieta
Capstone Major
Biology
Capstone College
Arts and Science
Audio/Visual Component
no
Capstone Prize Winner
yes
Won Capstone Funding
yes
Honors Categories
Sciences and Engineering
Subject Categories
Biology | Chemistry
Abstract
Vitamin B12 (B12)-based bioprobes were produced and investigated in vitro for studying the B12 uptake pathway in certain cancer cell lines and their target-specific capabilities as imaging and/or contrast agents in vivo. An intrinsic factor (IF)-bound B12 conjugate (B12-ReBQBA) was produced containing rhenium(I) for in vitro analysis of the cubilin-expressing placental choriocarcinoma cell line. B12-ReBQBA has millimolar toxicity in vitro and excitation and emission wavelengths in the visible region, 488 nm and 560 nm, respectively. The rhenium metal in this bioprobe can be readily exchanged for the metastable radioactive metal technetium-99m for in vivo single-positron emission computed tomography (SPECT) scanning. A second B12-based derivative was produced, incorporating a lanthanide metal in a bifunctional macrocycle, 1,4,7,10-tetraazacyclododecane-N,N¢,N¢¢,N¢¢¢-tetraacetic acid (DOTA). Europium(III) and terbium(III) were chelated by B12-enDOTA to produce target-specific imaging agents with unique fluorescent properties. These bioprobes, which excite in the UV region, are currently being tested on breast and pancreatic cancer cell lines, T-47D and MIA PaCa-2, respectively. Future work includes sensitizing the Eu(III) and Tb(III) bioprobes so they excite and emit light in the visible region.
Recommended Citation
Rabideau, Amy, "Production and In Vitro Investigation of Vitamin B12-Based Bioprobes" (2010). Honors Capstone Projects - All. 351.
https://surface.syr.edu/honors_capstone/351
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