Date of Award
Master of Science (MS)
Biomedical and Chemical Engineering
Hematopoietic growth factors, oligodendrocyte progenitor cells, stem cell factor and granulocyte colony-stimulating factor
Severe traumatic brain injury (TBI) causes severe damage in the white matter, leading to long-term disability and increased mortality. Demyelination, characterized by the damage of myelin sheaths and oligodendrocyte cell death, is a major pathological change in white matter injury after TBI, resulting in persistent neurological deficits. Previous studies have demonstrated that co-treatments with stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) (SCF+G-CSF) in the chronic phase of severe TBI improve neurological function recovery and enhance remyelination in the white matter. The SCF+G-CSF-enhanced remyelination is positively correlated with the proliferation of oligodendrocyte progenitor cells (OPCs) in the subventricular zone. However, it remains unclear whether SCF+G-CSF has a direct effect on OPCs to regulate OPC proliferation and differentiation. Using cultured primary OPCs, the findings of this thesis reveal that SCF+G-CSF can directly act on OPCs and promote the proliferation and differentiation of OPCs. The SCF+G-CSF-enhanced OPC proliferation is mediated by the NF-kB/cyclin D1 signaling pathway, and SCF+G-CSF promotes the differentiation of OPCs into oligodendrocytes in a time-dependent manner. These findings provide insights into SCF+G-CSF-enhanced remyelination during chronic TBI.
Li, Songruo, "Hematopoietic growth factors promote the proliferation and differentiation of oligodendrocyte progenitor cells" (2023). Theses - ALL. 698.