Date of Award
Master of Science (MS)
Biomedical and Chemical Engineering
Low frequency ultrasound has been used for therapeutic purposes since a long time. It is non-invasive and thus application for medical purpose is easier. This method has been used in labs for preclinical in-vitro settings to observe the effects of low frequency ultrasound sound on neoplastic cells lines. The human leukemia cell lines are sonic sensitive and when exposed to various low frequencies of ultrasound for an amount of time, can cause their lysis and decrease their viability. The project focuses on using low frequency ultrasound on syringe pump mediated flowing leukemia cell lines and see how much their sonic sensitivity varies when they are in motion in comparison to a control and when they are stationary. Tygon tubes are used to carry out the experiments and low frequency ultrasound is mediated by using a 20khz system at 30% amplitude. The cells were exposed to continuous sonication for varying time durations and varying flow rates and the end effects were noted. Various tests were performed to show the preferential damage of leukemia cell lines like apoptosis assays, actin staining and trypan blue exclusion. Based on the results obtained it showed that the sonic sensitivity is affected my motion and there is more damage to the neoplastic cells in comparison to when they are stationary sonicated. In future these studies will help to formulate a setup to analyze how much the sonic sensitivity will vary when the leukemia cells are mixed with whole blood cells and exposed to moving sonications. These studies may create a base for in-vivo experiments, as the results will give us an understanding about the exposure time which will cause maximum damage to the cells and how much time should the mouse be exposed to sonication to get preferential damage.
Raval, Dhairya, "Destruction of Moving Leukemia Cancer Cells In an Extra-Corporeal system using Continuous Low-frequency Ultrasound" (2018). Theses - ALL. 255.