Document Type

Honors Capstone Project

Date of Submission

Spring 5-1-2013

Capstone Advisor

Dr. Robert Doyle, Associate Professor

Honors Reader

Dr. James Hougland, Assistant Professor

Capstone Major

Biology

Capstone College

Arts and Science

Audio/Visual Component

no

Capstone Prize Winner

no

Won Capstone Funding

no

Honors Categories

Sciences and Engineering

Subject Categories

Biology

Abstract

Little is known about metal-citrate transport in Gram-positive bacteria. Previously, all such transporters identified have belonged to the citrate-metal hydrogen symport (CitMHS) family. Presented herein is our research on the predicted metal-citrate transport system in the Gram-positive bacteria Kineococcus radiotolerans. Given the high alkaline natural environment of this organism, it was hypothesized such a metal-citrate transporter may utilize an antiport mechanism, which to date has not been observed. Herein it is shown that K. radiotolerans has a calcium-citrate transporter. Preliminary results also support the hypothesis that this transporter operates through hydroxide antiport, rather than hydrogen symport, making this the first example of such transport for CitMHS reported to date. In future work we hope to confirm this and knock out the purported gene for this protein through use of a modified TargeTron system.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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Biology Commons

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