Honors Capstone Project
Date of Submission
Dr. Robert Doyle, Associate Professor
Dr. James Hougland, Assistant Professor
Arts and Science
Capstone Prize Winner
Won Capstone Funding
Sciences and Engineering
Little is known about metal-citrate transport in Gram-positive bacteria. Previously, all such transporters identified have belonged to the citrate-metal hydrogen symport (CitMHS) family. Presented herein is our research on the predicted metal-citrate transport system in the Gram-positive bacteria Kineococcus radiotolerans. Given the high alkaline natural environment of this organism, it was hypothesized such a metal-citrate transporter may utilize an antiport mechanism, which to date has not been observed. Herein it is shown that K. radiotolerans has a calcium-citrate transporter. Preliminary results also support the hypothesis that this transporter operates through hydroxide antiport, rather than hydrogen symport, making this the first example of such transport for CitMHS reported to date. In future work we hope to confirm this and knock out the purported gene for this protein through use of a modified TargeTron system.
Robertson, Eleanor, "Metal-Citrate Transport in the Gram-Positive Bacterium Kineococcus radiotolerans" (2013). Honors Capstone Projects - All. 71.
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