Degree Type

Honors Capstone Project

Date of Submission

Spring 5-1-2013

Capstone Advisor

Dr. Robert Doyle, Associate Professor

Honors Reader

Dr. James Hougland, Assistant Professor

Capstone Major


Capstone College

Arts and Science

Audio/Visual Component


Capstone Prize Winner


Won Capstone Funding


Honors Categories

Sciences and Engineering

Subject Categories



Little is known about metal-citrate transport in Gram-positive bacteria. Previously, all such transporters identified have belonged to the citrate-metal hydrogen symport (CitMHS) family. Presented herein is our research on the predicted metal-citrate transport system in the Gram-positive bacteria Kineococcus radiotolerans. Given the high alkaline natural environment of this organism, it was hypothesized such a metal-citrate transporter may utilize an antiport mechanism, which to date has not been observed. Herein it is shown that K. radiotolerans has a calcium-citrate transporter. Preliminary results also support the hypothesis that this transporter operates through hydroxide antiport, rather than hydrogen symport, making this the first example of such transport for CitMHS reported to date. In future work we hope to confirm this and knock out the purported gene for this protein through use of a modified TargeTron system.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

Included in

Biology Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.