antineoplastic agent; carbonic acid; carboplatin; nitrogen 15; platinum; article; chemical analysis; chemical modification; controlled study; culture medium; drug determination; drug structure; human; human cell; leukemia cell line; nuclear magnetic resonance spectroscopy
Using [1H,15N] heteronuclear single quantum coherance (HSQC) NMR and 15N-labeled carboplatin, 1, we show that Jurkat cells affect the rate of disappearance of the HSQC NMR peak in culture medium for this Pt2+ anticancer drug. The decay or disappearance rate constant for 1 in culture medium containing cells is k1 = kc [CO32 -] + km + ku N, where kc is the rate constant for reaction of 1 with carbonate in the medium, km is the rate constant for reaction of 1 with all other components of the medium, and ku is the rate constant for reaction of 1 with cells having a number density N in the medium. Since Jurkat cells only take up a small amount of the platinum present in the medium (
Di Pasqua, Anthony J.; Goodisman, Jerry; Kerwood, Deborah J.; Toms, Bonnie B.; and Dabrowiak, James C., "Modification of Carboplatin by Jurkat Cells" (2007). Chemistry Faculty Scholarship. 30.
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