Date of Award
Master of Science (MS)
Biomedical and Chemical Engineering
Cardiomyocyte, Dynamic substrate, Focal adhesion, Mechanobiology, Shape memory polymer
Focal adhesions are the large molecular complexes that anchor cells to the ECM, which is involved in cell spreading, migration, and proliferation. In our previous work, the study of cardiomyocyte morphological changes and myofibril remodeling, responding to the dynamic nano-topographic substrate, have been accomplished. The results showed focal adhesion experienced disassembly and reassembly in the first 12 hours. In this study, the hiPSC-CMs were seeded on the SMP substrates with the flat-to-wrinkle dynamic surface. Paxillin, vinculin, and zyxin from different layers of focal adhesive architectures were co-stained with F-actin. The fluorescent images were analyzed to investigate the details of focal adhesions remodeling and the dynamics of costameres in the first stage post triggering the substrate wrinkle formation.
Shi, Huaiyu, "Cell-adhesion Activities Responding to Nano-Dynamic Substrate" (2020). Theses - ALL. 461.