Date of Award
August 2020
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Biomedical and Chemical Engineering
Advisor(s)
Zhen Ma
Keywords
Cardiomyocyte, Dynamic substrate, Focal adhesion, Mechanobiology, Shape memory polymer
Subject Categories
Engineering
Abstract
Focal adhesions are the large molecular complexes that anchor cells to the ECM, which is involved in cell spreading, migration, and proliferation. In our previous work, the study of cardiomyocyte morphological changes and myofibril remodeling, responding to the dynamic nano-topographic substrate, have been accomplished. The results showed focal adhesion experienced disassembly and reassembly in the first 12 hours. In this study, the hiPSC-CMs were seeded on the SMP substrates with the flat-to-wrinkle dynamic surface. Paxillin, vinculin, and zyxin from different layers of focal adhesive architectures were co-stained with F-actin. The fluorescent images were analyzed to investigate the details of focal adhesions remodeling and the dynamics of costameres in the first stage post triggering the substrate wrinkle formation.
Access
Open Access
Recommended Citation
Shi, Huaiyu, "Cell-adhesion Activities Responding to Nano-Dynamic Substrate" (2020). Theses - ALL. 461.
https://surface.syr.edu/thesis/461
