Document Type
Article
Date
2-1-1997
Keywords
Chemistry
Disciplines
Chemistry
Description/Abstract
The fluorescence of ethidium bromide (EB) bound to equimolar amounts of supercoiled form I and unstrained linear form III pBR322, SV40 and PM2 DNA in agarose gels has been measured by scanning a photographic negative of the gel with a microdensitometer. For SV40 and PM2 DNA, commonly used staining conditions cause both forms, i.e. linear and supercoiled, to fluoresce to the same extent. This obviates the need to use a correction factor for the fluorescence of form I DNA when measuring the amount of this form relative to the amounts of unstrained forms in agarose gels. In the case of PBR322 DNA, form I was found to fluoresce | 20% more than form III DNA.
Recommended Citation
Shubsda, Michael F.; Goodisman, Jerry; and Dabrowiak, James C., "Quantitation of ethidium-stained closed circular DNA in agarose gels" (1997). Chemistry - All Scholarship. 93.
https://surface.syr.edu/che/93
Source
local input
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Additional Information
Copyright 1997 Journal of Biochemical and Biophysical Methods. This article may be downloaded for personal use only. Any other use requires prior permission of the author and Journal of Biochemical and Biophysical Methods.
The article may be found at http://www.sciencedirect.com/science/article/pii/S0165022X96012043