Document Type

Article

Date

2-1-1997

Embargo Period

4-29-2013

Keywords

Chemistry

Disciplines

Chemistry

Description/Abstract

The fluorescence of ethidium bromide (EB) bound to equimolar amounts of supercoiled form I and unstrained linear form III pBR322, SV40 and PM2 DNA in agarose gels has been measured by scanning a photographic negative of the gel with a microdensitometer. For SV40 and PM2 DNA, commonly used staining conditions cause both forms, i.e. linear and supercoiled, to fluoresce to the same extent. This obviates the need to use a correction factor for the fluorescence of form I DNA when measuring the amount of this form relative to the amounts of unstrained forms in agarose gels. In the case of PBR322 DNA, form I was found to fluoresce | 20% more than form III DNA.

Additional Information

Copyright 1997 Journal of Biochemical and Biophysical Methods. This article may be downloaded for personal use only. Any other use requires prior permission of the author and Journal of Biochemical and Biophysical Methods.

The article may be found at http://www.sciencedirect.com/science/article/pii/S0165022X96012043

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Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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