Cellular respirations; Concentration dependents; Glutathione; In cells; Mesoporous silica nanoparticles; Respiration rates; Submitochondrial particles; Time dependents
We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.
Tao, Zhimin; Asefa, Tewodros; Duncan, Cole; Anan, Abhishek; and Goodisman, Jerry, "Mesoporous Silica Nanoparticles Inhibit Cellular Respiration" (2008). Chemistry Faculty Scholarship. 32.
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.