Document Type
Article
Date
2-25-1988
Keywords
congocidine; dactinomycin; deoxyribonuclease I; DNA; restriction endonuclease; article; DNA base composition; kinetics; metabolism; nucleic acid analysis; nucleotide sequence; plasmid
Disciplines
Chemistry
Description/Abstract
Footprinting experiments for DNase I digests of a 139-base-pair segment of pBR-322 DNA in the presence of either netropsin or actinomycin D were carried out. Plots of oligonucleotide concentration as a function of drug concentration were analyzed to study the enhancement in cleavage rates at approximately 30 sites, accompanying drug binding at other sites. The pattern of enhancements is not consistent with drug-induced DNA structural changes, but agrees with a redistribution mechanism involving DNase I. Since the total number of enzyme molecules per fragment remains unchanged, drug binding at some sites increases the enzyme concentration at other sites, giving rise to increased cleavage. The consequences of the redistribution mechanism for analysis of footprinting experiments are indicated.
Recommended Citation
Ward, Brian; Rehfuss, Robert; Goodisman, Jerry; and Dabrowiak, James C., "Rate Enhancements in the DNase I Footprinting Experiment" (1988). Chemistry - All Scholarship. 13.
https://surface.syr.edu/che/13
Source
local input
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Additional Information
Copyright 1988 Nucleic Acids Research. This article may be downloaded for personal use only. Any other use requires prior permission of the author and Nucleic Acids Research.
The article may be found at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC336320/