Document Type

Article

Date

10-2007

Embargo Period

4-29-2013

Keywords

Chemistry

Disciplines

Chemistry

Description/Abstract

Using [1H,15N] heteronuclear single quantum coherance (HSQC) NMR and 15N-labeled carboplatin, 1, we show that Jurkat cells affect the rate of disappearance of the HSQC NMR peak in culture medium for this Pt2+ anticancer drug. The decay or disappearance rate constant for 1 in culture medium containing cells is k1=kc[CO32-]+km+kuN, where kc is the rate constant for reaction of 1 with carbonate in the medium, km is the rate constant for reaction of 1 with all other components of the medium, and ku is the rate constant for reaction of 1 with cells having a number density N in the medium. Since Jurkat cells only take up a small amount of the platinum present in the medium (<1%), the observed disappearance of the HSQC NMR peak for 1 cannot be due to uptake of carboplatin by the cells.

Additional Information

Copyright 2007 Journal of Inorganic Biochemistry. This article may be downloaded for personal use only. Any other use requires prior permission of the author and Journal of Inorganic Biochemistry.

The article may be found at http://www.sciencedirect.com/science/article/pii/S0162013407001432

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