Title

Site-Specific Binding Constants for Actinomycin D on DNA Determined from Footprinting Studies

Author(s)/Creator(s)

Jerry Goodisman, Syracuse UniversityFollow
Robert Rehfuss, Syracuse University
Brian Ward, Syracuse University
James C. Dabrowiak, Syracuse UniversityFollow

Document Type

Article

Date

9-17-1991

Keywords

Chemistry

Disciplines

Chemistry

Description/Abstract

We report site-specific binding constants for the intercalating anticancer drug actinomycin D (Act-D), binding to a 139-base-pair restriction fragment from pBR 322 DNA. The binding constants are derived from analysis of footprinting experiments, in which the radiolabeled 139-mer is cleaved using DNase I, the cleavage products undergo gel electrophoresis, and, from the gel autoradiogram, spot intensities, proportional to amounts of cleaved fragments, are measured. A bound drug prevents DNase I from cleaving at -7 bonds, leading to decreased amounts of corresponding fragments. With the radiolabel on the 3’ end of the noncoding strand (A-label), we measured relative amounts of 54 cleavage products at 25 Act-D concentrations. For cleavage of the 139-mer with the label on the 3’ end of the coding strand (G-label), relative amounts of 43 cleavage products at 11 Act-D concentrations were measured. These measurements give information about - 120 base pairs of the restriction fragment (- 12 turns of the DNA helix); in this region, 14 strong and weak Act-D binding sites were identified. The model used to interpret the footprinting plots is derived in detail. Binding constants for 14 sites on the fragment are obtained simultaneously. It is important to take into account the effect of drug binding at its various sites on the local concentration of probe elsewhere. It is also necessary to include in the model weak as well as strong Act-D sites on the carrier DNA which is present, since the carrier DNA controls the free-drug concentration. As expected, the strongest sites are those with the sequence (all sequences are 5’ - 3’) GC, with TGCT having the highest binding constant, 6.4 X lo6 M-l. Sites having the sequence GC preceded by G are weak binding sites, having binding constants approximately 1 order of magnitude lower than those of the strong sites. Also, the non-GC-containing sequences CCG and CCC bind Act-D with a binding constant comparable to those of the weak GGC sites. The analysis may reveal drug-induced structural changes on the DNA, which are discussed in terms of the mechanism of Act-D binding.

Additional Information

Reprinted with permission from Goodisman, J., Rehfuss, R., Ward, B., & Dabrowiak, J. C. (1992). Site-specific binding constants for actinomycin D on DNA determined from footprinting studies. Biochemistry®, 31(4), 1046-1058. Copyright 1991 American Chemical Society.

http://pubs.acs.org/doi/pdf/10.1021/bi00119a013

Recommended Citation

Goodisman, J., Rehfuss, R., Ward, B., & Dabrowiak, J. C. (1992). Site-specific binding constants for actinomycin D on DNA determined from footprinting studies. Biochemistry®, 31(4), 1046-1058.

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This work is licensed under a Creative Commons Attribution 3.0 License.