Document Type

Article

Date

2-25-1988

Embargo Period

2-6-2013

Keywords

congocidine; dactinomycin; deoxyribonuclease I; DNA; restriction endonuclease; article; DNA base composition; kinetics; metabolism; nucleic acid analysis; nucleotide sequence; plasmid

Disciplines

Chemistry

Description/Abstract

Footprinting experiments for DNase I digests of a 139-base-pair segment of pBR-322 DNA in the presence of either netropsin or actinomycin D were carried out. Plots of oligonucleotide concentration as a function of drug concentration were analyzed to study the enhancement in cleavage rates at approximately 30 sites, accompanying drug binding at other sites. The pattern of enhancements is not consistent with drug-induced DNA structural changes, but agrees with a redistribution mechanism involving DNase I. Since the total number of enzyme molecules per fragment remains unchanged, drug binding at some sites increases the enzyme concentration at other sites, giving rise to increased cleavage. The consequences of the redistribution mechanism for analysis of footprinting experiments are indicated.

Additional Information

Copyright 1988 Nucleic Acids Research. This article may be downloaded for personal use only. Any other use requires prior permission of the author and Nucleic Acids Research.

The article may be found at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC336320/

Source

local input

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Included in

Chemistry Commons

Share

COinS